Studies on gynaecological hydrophilic lactic acid preparations, Part 7: use of chitosan as lactic acid carrier in intravaginal tablets (globuli vaginales).

Intravaginal tablets based on hydrophilic methylcellulose and containing lactic acid complexed with chitosan undergo deformation under standard conditions. The high flow--limit of the gel originating from the tablets as well as its dynamic viscosity should ensure the durability of this dosage form on the vaginal mucosa. By selecting either ratios of lactic acid to chitosan of 1:1 or 2:1 it is possible to obtain tablets that disintegrate into a gel form at pH 3.8-4.4, i.e. the pH remains within the physiological range. Increasing the amount of lactic acid in the complex in relation to polymer to a 4:1 ratio results in gels with a lower pH while giving an acid reserve that can neutralize the excess of alkali present in severe vaginal infections.

Studies on gynaecological hydrophilic lactic acid preparations, part 6: use of Eudragit E-100 as lactic acid carrier in intravaginal tablets.

Hydrophilic intravaginal tablets based on methylcellulose and containing lactic acid component with Eudragit E-100 undergo swelling in standard conditions. A high flow-limit of the gel that originates from the tablets as well as its dynamic viscosity should allow for the durable dosage form in the vagina. By choosing a 1:1 ratio of lactic acid to Eudragit E-100, it is possible to obtain tablets disintegrating into a gelform at physiological range of 3.8-4.4. An increase in the amount of lactic acid in the complex in relation to the polymer up to 2:1 and 3:1 ratios results in gels with a lower pH. These gels possess an acid reserve that might be ableto neutralise the excess of alkali present in severe vaginal infections.

Studies on gynaecological hydrophilic lactic acid preparations. Part 5: The use of Eudragit E-100 as lactic acid carrier in intravaginal tablets.

Intravaginal tablets based on hydrophilic methylcellulose and containing lactic acid complexed with Eudragit E-100 undergo deformation under standard conditions. The high flow--limit of gel originating from the tablets as well as its dynamic viscosity should enable durability of this dosage form on the vaginal mucosa. By selecting either 1 : 1 or 2 : 1 ratios of lactic acid to Eudragit E-100 it is possible to obtain tablets that disintegrate into a gelform at pH 3.8-4.4, i.e. the pH remains within the physiological range. Increasing the amount of lactic acid in the complex in relation to the polymer to a 4:1 ratio results in gels with a lower pH while possessing an acid reserve that can neutralize the excess of alkali present in severe vaginal infections.

Studies on gynaecological hydrophilic lactic acid preparations. Part 4: Effects of polyvinyl pyrrolidone K-90 on properties of methylcellulose gels.

In continuing the investigation of the binding of lactic acid with basic polymers polyvinyl pyrrolidone K-90 (PVP) was used. Use of PVP in mixtures with lactic acid in experimentally determined and calculated proportions allowed the preparation of gels with pH 3.8-4.4, i.e. maintaining the physiological conditions in the vagina. The viscosity of the methylcellulose gels increases proportionally to the amount of the PVP--lactic acid complex added. Examination of the gels in the biopharmaceutical model, which simulated the physiological conditions of the vagina, allowed determination of the mobility of the preparations in patients.

[Application of 2% clindamycin cream in the treatment of bacterial vaginosis and valuation of methylcellulose gel containing the complex of Chitosan F and PVP k-90 with lactic acid as carrier for intravaginally adhbited medicines in the cases of pregnancies with the symptoms of preterm delivery]. / Zastosowanie 2% kremu z klindamycyna w leczeniu bakteryjnej waginozy oraz ocena zelu z metylocelulozy, zawierajacego kompleks Chitiozanu F i PVP K-90 z kwasem mlekowym, jako nosnika dla leków stosowanych dopochwowo w przypadkach ciezarnych z objawami porodu przedwczesnego zagrazajacego.

OBJECTIVES: There are many reports informing about the connection between BV and the increased risk of preterm delivery. The reason of self-concession and reversion of BV after having executed an efficient treatment has not yet been properly explained. DESIGN: The aim of this work was the clinical valuation of the 2% Clindamycin cream in the treatment of BV and of the methylcellulose gel containing the complex of Chitosan F and PVP K-90 with lactic acid as a carrier for intravaginally adhbited medicines in the cases of pregnancies with the symptoms of a preterm delivery. MATERIAL AND METHODS: The research comprised 145 pregnant between 24-34 week of pregnancy, hospitalised because of the symptoms of a preterm menace delivery. In the case of the detection of BV, a 10-day therapy using intravaginal cream containing 2% Clindamycin was executed. In the cases not qualified as BV, the methylcellulose gel containing the complex of Chitosan F and PVP K-90 with lactic acid has been intravaginally adhibited for 10 days. CONCLUSIONS: 1. Application of 2% Clindamycin cream is an efficient method of the treatment of bacterial vaginosis. 2. Methylcellulose gel containing lactic acid combined with the complex of Chitosan F and PVP K-90 allows a persistent maintenance of the correct pH of vagina. 3. Methylcellulose gel, because of its physico-chemical properties similar to physiological mucus, is a universal carrier for intravaginally adhibited medicines.

Studies on gynaecological hydrophilic lactic acid preparations. Part 3: Effects of chitosan on the properties of methylcellulose gels.

Methylcellulose gels comprising lactic acid, similarly to its water solutions, have pH values between 2.0 and 2.6, i.e. out of the physiological range. Addition of Chitosan enables preparation of gels with pH values from 2.2 to 4.9 similar to the natural vagina pH range, 3.8-4.4. The absolute viscosity of the gels, ranging from 27 up to 700 mPa.s, depends on chemical composition and on excess of lactic acid used. Measurement of adhesion enabled the selection of gels which remain located at the point of application under simulated biopharmaceutical conditions. The behavior of those preparations in the true environment of the vagina needs to be verified in vivo.

Studies on dressing for dental surgery use. Part 2. Influence of Kunitz' protease inhibitor on properties of dental gelatinous dressings.

To increase resistance of gelatinous dressings to the etching action of trypsin in vitro, the Kunitz' protease inhibitor has been added. It has been found that the etching rate of the dressings was reduced in proportion to the inhibitor added. Addition of plasticizers in the presence of an inhibitor reduces the etching rate of the dressings and the effect depends on the type and concentration of the plasticizer added. Thermally sterilized dressings have an average of 10% less resistance to the etching action of trypsin. The liberation of the inhibitor from the dressings follows first order kinetics, whereas its liberation rate depends upon the type of the plasticizer used.